Developing buffer for ELISPOT assay
1X PBS (10X PBS [Roche] diluted to 1X with H2O)
0.05% Tween 20
10 g/L bovine serum albumin (BSA)
0.5 μg/mL mouse horseradish peroxidase (HRP)-conjugated anti-fluorescein isothiocyanate (FITC) IgG monoclonal antibody (mAb) (Cedarlane Laboratories)
0.5 μg/mL streptavidin alkaline phosphatase (Cedarlane Laboratories)
