Recipe

HMT reaction buffer

S-Adenosyl-L-[methyl-3H] methionine (0.03 mCi/mL; PerkinElmer)

DTT (dithiothreitol; 0.5 mM)

EDTA (4 mM)

PMSF (phenylmethylsulfonyl fluoride; 1 mM)

Tris-Cl (20 mM, pH 8.0)

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  1. doi:10.1101/pdb.rec12338 Cold Spring Harb Protoc 2010: pdb.rec12338-
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