Array tomography subbing solution
| Reagent | Amount to add (for 300 mL) | Final concentration |
|---|---|---|
| Chromium (III) potassium sulfate dodecahydrate (Sigma-Aldrich 243361) | 0.15 g | 0.05% |
| Gelatin from porcine skin, Type A, ~300 g Bloom (Sigma-Aldrich G1890) | 1.5 g | 0.5% |
| H2O | 300 mL | |
| Dissolve the chromium potassium sulfate in 10 mL of H2O. Dissolve the gelatin in 290 mL of H2O by heating to <60°C. Cool to ~37°C. Add the chromium potassium sulfate to the cooled gelatin solution. Filter and pour into a staining tank. | ||
| Prepare the day of use. | ||
