Array tomography blocking solution with NGS
| Reagent | Amount to add (for 1 mL) | Final concentration |
|---|---|---|
| Normal goat serum (NGS; Invitrogen PCN5000)a | 100 μL | 10% |
| Tris-buffered saline (from tablets; Sigma-Aldrich T5030) | 800 μL | |
| Tween 20 (1%) (Electron Microscopy Sciences 25564)b | 100 μL | 0.1% |
| Prepare the day of use. | ||
| aNGS can be stored as frozen aliquots for several months. | ||
| bThe 1% Tween stock can be stored for several months at 4°C. | ||
