Table 1. Suggested working parameters for disruption of cells using nitrogen cavitation

Cell type	Cell suspension	Pounds per sq in (psi)	Cells used/mL	No. times through	Cells remaining/mL	Nuclei remaining/mL	Percent (%) cells totally lysed
Cultured cells	KB	500	3.4 × 10⁶	1	2.8 × 10⁵	n.d.	93
	KB	500		2	2.0 × 10³	n.d.	99.95
	KB	250	3.3 × 10⁶	1	5.5 × 10⁵	4.62 × 10⁵	69.4
	KB	250		2	5.4 × 10⁴	2.2 × 10⁴	97.7
	KB	250		3	0	0	100
	KB	0	1.8 × 10⁶	0	1.08 × 10⁶	7.6 × 10⁵	--
	KB	250		1	2.4 × 10⁵	6.4 × 10⁵	52.2
	KB	250		2	3.4 × 10⁴	5.0 × 10⁴	95.4
	KB	250		3	1.6 × 10⁴	4.6 × 10⁴	96.6
Tissue	Rat liver	500	9.2 × 10⁵	1	8.5 × 10⁵	n.d.	7.8
		500		2	0	--	100
Blood	Chicken red blood cells	1000	5.6 × 10⁹	1	9.6 × 10⁸	n.d.	83
Bacteria	Escherichia coli	1500	1.3 × 10¹⁰	1	1.2 × 10¹⁰	n.d.	8
		1500		2	6.6 × 10⁹	n.d.	50
	E. coli	1500	6.5 × 10⁸	1	3.6 × 10⁸	n.d.	45
		1500		2	3.3 × 10⁸	n.d.	50
Parameters modified from Kimble-Chase Instruction Sheet 8814551, with values determined using a Mini-Bomb Cell Disruptcion Chamber.
(KB cells) Human oral epidermoid carcinoma.
(n.d.) Not determined.