Visualizing the microtubule-based transport of Golgi-derived vesicles in the axon of a stage 4 hippocampal neuron
Movie 30_3.
Cells were cotransfected with a GFP-tagged membrane protein (NgCAM) and an mCherry construct targeted to the lumen of vesicles in the default constitutive secretory pathway. Images were acquired continuously every 600 msec for 60 sec. Movie, 4.6 sec; total time, 60 sec; play rate, x13. [Supplemental movie from Imaging in Developmental Biology: A Laboratory Manual, Cold Spring Harbor Laboratory Press.]
