Three-dimensional rotation of tiled, stitched z-stack. Tg(Tie1:H2B::eYFP) quail embryo somites, HH 12-13, near somite 10
Movie 41_1.
Images were collected on a Zeiss META 510 two-photon microscope. Tiles were stitched using Zeiss LSM software with 10% overlap in x-y. The stitched stack was animated using Bitplane’s Imaris 5.7.2 software. Objective, LD-Apochromat 40x/1.1 W Korr objective; stack size, 427.5 × 630.1 × 126 μm; tiled stacks, 2 × 3 tiles; excitation, 860 nm (23%); emission, 505LP; initial frame, anterior on left, posterior on right. [Supplemental movie from Imaging in Developmental Biology: A Laboratory Manual, Cold Spring Harbor Laboratory Press.]
