Stacking gel mixture

Agarose

0.5 M tricine-NaOH (pH 10)

10% CTAB (cetyltrimethylammonium bromide)

Add 35 mg of agarose to 1.25 mL of 0.5 M tricine-NaOH (pH 10) and 50 μL of 10% CTAB. Add H₂O to 5 mL. Melt the agarose by boiling or microwaving the mixture before pouring the gel. This makes enough gel mixture to pour stacking gels for two minigels, 1.5-mm thick.

The final mixture is 0.7% agarose, 0.1% CTAB, 125 mM tricine-NaOH (pH 10).