Tricine running buffer (5X)

Tricine

CTAB (cetyltrimethylammonium bromide)

1 M arginine (free base) in H₂O

Dissolve 22.4 g of tricine and 5 g of CTAB in 75 mL of 1 M arginine. Add H₂O to a final volume of 1 L. The pH of the buffer should be ~8.2; no pH adjustment is required. Store the running buffer at room temperature and dilute to 1X before use.