| Animal tissues |
AEBSF (0.2 mM) (or DCI [0.1 mM] or PMSF [0.2 mM]) |
Serine |
20 mM in methanol (DCI: 10 mM in DMSO; PMSF: 200 mM in ethanol or isopropanol) |
|
Benzamidine (1 mM) |
Serine |
100 mM |
|
Leupeptin (10 μg/mL) |
Serine/cysteine |
1 mg/mL |
|
Pepstatin (10 μg/mL) |
Aspartic |
5 mg/mL in methanol |
|
Aprotinin (trasylol) (1 μg/mL) |
Serine |
0.1 mg/mL |
|
EDTA or EGTAc (1 mM)
|
Metallo |
100 mM |
| Plant tissues |
AEBSF (0.2 mM) (or DCI [0.1 mM] or PMSF [0.2 mM]) |
Serine |
20 mM in methanol (DCI: 10 mM in DMSO; PMSF: 200 mM in ethanol or isopropanol) |
|
Chymostatin (20 μg/mL) |
Serine/cysteine |
1 mg/mL in DMSO |
|
EDTA or EGTAc (1 mM)
|
Metallo |
100 mM |
| Yeasts and fungi |
AEBSF (0.2 mM) (or DCI [0.1 mM] or PMSF [0.2 mM]) |
Serine |
20 mM in methanol (DCI: 10 mM in DMSO; PMSF: 200 mM in ethanol or isopropanol) |
|
Pepstatin (15 μg/mL) |
Aspartic |
5 mg/mL in methanol |
|
1,10-phenanthroline (5 mM) |
Metallo |
1 M in ethanol |
| Bacteria |
AEBSF (0.2 mM) (or DCI [0.1 mM] or PMSF [0.2 mM]) |
Serine |
20 mM in methanol (DCI: 10 mM in DMSO; PMSF: 200 mM in ethanol or isopropanol) |
|
EDTA or EGTAc (1 mM)
|
Metallo |
100 mM |
|
| (Reprinted from Simpson 2003; adapted from North 1989.) |
| Abbreviations: (AEBSF) 4-(2-aminoethyl)-benzenesulfonylfluoride; (DCI) 3,4-dichloroisocoumarin; (DMSO) dimethylsulfoxide;
(EDTA) ethylenediamine tetraacetic acid; (EGTA) ethylene glycol bis (β-aminoethyl ether) N,N,N’,N’-tetraacetic acid; (PMSF) phenylmethylsulfonyl fluoride.
|
| Mr values of inhibitors: AEBSF (240); PMSF (174); DCI (215); EDTA (disodium salt, dihydrate) (372); benzamidine (hydrochloride)
(157); leupeptin (427); pepstatin (686); aprotinin (6500); chymostatin (605); 1,10-phenanthroline (198).
|
| aFor a review of proteolytic enzymes, see Perlmann and Lorand (1970); Neurath (1989).
|
| bAqueous solution unless otherwise indicated.
|
| cAn efficient chelator of divalent metal cations other than Mg2+ (for which it has a 103-fold lower affinity) (Gegenheimer 1990).
|
