| AEBSF |
<1 mM |
20 mM |
239.5 mg/10 mL H2O (100 mM)
|
4 mL |
Serine proteases |
| EDTA |
1-10 mM |
100 mM |
19 mg/100 mL H2O (0.5 M)
|
4 mL |
Metallo proteases |
| Leupeptin |
10-100 μM |
2 mM |
18.9 mg/2 mL H2O (20 mM)
|
2 mL |
Cysteine/serine proteases |
| Pepstatin |
1 μM |
100 μM |
6.8 mg/10 mL methanol (1 mM) |
2 mL |
Aspartic proteases |
|
| (Reprinted from Simpson 2003; data, in part, from Calbiochem 1998.) |
| aMix the inhibitor solutions and bring to a final volume of 20 mL with H2O or appropriate aqueous buffer. The resulting solution, 20 mL of a stock 100X protease inhibitor cocktail, can be aliquoted
into microcentrifuge tubes and stored at −20ºC until required.
|
