Recipe

SDS solubilization buffer (10X)

Reagent Quantity
(for 500 mL) 		Final concentration
EDTA (500 mM, pH 8.0) 10 mL 10 mM
SDS (sodium dodecyl sulfate; 10%) 250 mL 5%
Tris-Cl (1 M, pH 7.5) 100 mL 200 mM
H2O, RNase-free 140 mL --
Store indefinitely at room temperature.
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  1. doi:10.1101/pdb.rec12226 Cold Spring Harb Protoc 2010: pdb.rec12226-
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