Recipe

TBE electrophoresis buffer (10X)

Reagent Quantity (for 1 L) Final concentration
Tris base 121.1 g 1 M
Boric acid 61.8 g 1 M
EDTA (disodium salt) 7.4 g 0.02 M
Prepare with RNase-free H2O. Dilute 100 mL to 1 L to make gel running buffer. Store for up to 6 mo at room temperature.
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  1. doi:10.1101/pdb.rec12231 Cold Spring Harb Protoc 2010: pdb.rec12231-
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