Recipe

Binding buffer for mRNA

Reagent Quantity (for 500 mL) Final concentration
LiCl (5 M) 50 mL 0.5 M
Elution buffer for mRNA (10X) 50 mL 1X
H2O, RNase-free 400 mL
All reagents used to make this buffer should be prepared with RNase-free H2O.
Store for up to 1 yr at room temperature.
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  1. doi:10.1101/pdb.rec12250 Cold Spring Harb Protoc 2010: pdb.rec12250-
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