Recipe

Ammonium carbonate buffer

Ammonium carbonate (7.85 g/L, pH 9.2) (ice-cold)

Pepstatin (3 mg/L)

PMSF (phenylmethylsulfonyl fluoride) (22 mg/L)

Add the pepstatin and PMSF with adequate stirring just before use.

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  1. doi:10.1101/pdb.rec12252 Cold Spring Harb Protoc 2010: pdb.rec12252-
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