Recipe

HEPES buffer

Reagent Amount to add (for 1 L) Final concentration
NaCl 6.72 g 115 mM
CaCl2 133 mg 1.2 mM
MgCl2 114 mg 1.2 mM
K2HPO4 418 mg 2.4 mM
HEPES 4.77 g 20 mM
H2O to 1 L
Adjust the pH to 7.4 with HCl or NaOH. HEPES buffer can be stored refrigerated for several weeks.
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  1. doi:10.1101/pdb.rec12259 Cold Spring Harb Protoc 2010: pdb.rec12259-
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