Recipe

Block solution (D)

Reagent Quantity (for 5 mL) Final concentration
PBSBTX 5 mL 1X
Normal goat seruma 5 μL 0.1% (v/v)
Glycine (1 M in phosphate-buffered saline [PBS] containing 2% sodium azide) 50 μL 1% (w/v)
Prepare fresh and keep cold.
aOmit if using anti-goat antibodies.
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  1. doi:10.1101/pdb.rec12271 Cold Spring Harb Protoc 2010: pdb.rec12271-
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