Recipe

Hybridization buffer (D)

Reagent Quantity (for 50 mL) Final concentration
Formamide, deionized 25 mL 50% (v/v)
SSC (20X) 10 mL 4X
Tween 20 (10%, v/v) 0.5 mL 0.1% (v/v)
Heparin (50 mg/mL) 50 μL 0.05 mg/mL
RNase-free H2O 14.5 mL
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  1. doi:10.1101/pdb.rec12274 Cold Spring Harb Protoc 2010: pdb.rec12274-
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