Electroporation buffer with tricaine

1.8 mM CaCl₂

5 mM HEPES (pH 7.2)

5 mMKCl

180 mM NaCl

0.017% tricaine (Western Chemical MS-222)

The electroporation buffer recipe is from Cerda et al. (Cerda GA, Thomas JE, Allende ML, Karlstrom RO, Palma V. 2006. Electroporation of DNA, RNA, and morpholinos into zebrafish embryos. Methods 39: 207-211).