Recipe

Agarose solution (0.5%)

Agarose, low gelling temperature (Sigma A9414)

Electroporation buffer with tricaine

Make a solution of 0.5% agarose in electroporation buffer with tricaine. Dissolve the agarose by microwaving. Place the solution in an incubator preset to 32°C and allow the temperature to equilibrate.

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  1. doi:10.1101/pdb.rec12279 Cold Spring Harb Protoc 2010: pdb.rec12279-
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