Recipe

High-potassium HL-3 saline

Reagent Quantity (for 1 L) Final concentration
NaCl 2.34 g 40 mM
KCl 6.71 g 90 mM
MgCl2•6H2O 4.07 g 20 mM
NaHCO3 0.84 g 10 mM
Sucrose 1.71 g 5 mM
Trehalose 1.89 g 5 mM
HEPES 1.19 g 5 mM
Dissolve the reagents in H2O. Bring the pH to 7.2 and adjust the final volume to 1 L with H2O.
Add CaCl2 from a 1 M stock solution to give the desired concentration of Ca2+.
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  1. doi:10.1101/pdb.rec12285 Cold Spring Harb Protoc 2010: pdb.rec12285-
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