Table

Table 1. Physiological parameters monitored during surgery and two-photon fluorescence imaging of cerebral dynamics in rat

Troubleshooting
Physiological parameter Typical valuea Problem Solution
Intracranial pressure 3.7-4.4 mm Hg High Too much trauma to the brain. Start again.
Low Stop potential leak of cerebrospinal fluid.
Arterial blood pressureb 150/90 mm Hg High Encourage urination by massaging bladder, or increase anestheticc dose (if under-anesthetized).
Low Increase blood volume by injecting saline, or decrease anestheticc dose (if over-anesthetized).
Temperature 37°C-38°C Low Use hand warmer, hair dryer or heat lamp.
Respiratory pressure 10-15 cm H2O High Check for obstruction of the tracheal tube or decrease the flow rate.
Low Increase the flow rate.
pHd
7.35-7.45 Low Administer sodium bicarbonate (NaHCO3) (IV) according to base excess (ABEc): If ABEc < -5 mmol/L, then administer a number of mL of 1 M NaHCO3 equal to (|ABEc|/10).
Heart rate 320-450 bpm High Increase anestheticc dose.
Low Decrease anestheticc dose.
Oxygen saturation 96%-99% Low Increase the fraction of inspired oxygen (FiO2) or decrease anestheticc dose.
pCO2 30-36 mm Hg High Increase respiratory rate.
Low Decrease respiratory rate.
aTypical values are found in Waynforth and Flecknell (1994), Flecknell (1996), Hutchinson et al. (2006), and Nagra et al. (2009).
bAbsolute range of arterial blood pressure depends on the level of anesthesia.
cAssuming a halogenated anesthetic (e.g., isoflurane).
dAs measured by arterial blood gas analysis.
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  1. doi:10.1101/pdb.tab195494 Cold Spring Harb Protoc 2010: pdb.tab195494-

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