Table 2. Fusions between MBPs and GFP or other FPs
| MBP family | MBP species (individual gene or protein name) | Species/cell line expressing GFP fusion | Type of transfection | References (spectral variant if relevant) |
|---|---|---|---|---|
| APC | Human (APC) | SW480, COS7 | Transient, stable | Langford et al. 2006; Li et al. 2008 |
| Xenopus (APC) | Xenopus A6 cells | Transient | Mimori-Kiyosue et al. 2000a, 2007 | |
| CLIP-170 | Mammalian (CLIP-170) | Vero, CHO, NRK, COS-7 | Transient, stable | Perez et al. 1999; Komarova et al. 2002, 2005, 2009; Folker et al. 2005 (all YFP) |
| Mammalian (CLIP-170) | Transgenic mice | Akhmanova et al. 2005 | ||
| Mammalian (CLIP-170) | Cowpea mesophyll protoplasts, tobacco BY-2 | Transient, stable | Dhonukshe and Gadella 2003 (YFP) | |
| Saccharomyces cerevisiae (BIK1) | S. cerevisiae | Stable | Lin et al. 2001; Caudron et al. 2008 | |
| Schizosaccharomyces pombe (TIP1) | S. pombe | Stable | Brunner and Nurse 2000; Niccoli and Nurse 2002; Zimmerman and Chang 2005 (last two YFP) | |
| ClipR-59 |
Mammalian (CLIPR-59) | HeLa | Transient | Perez et al. 2002 |
| CLASPS/MAST/ ORBIT |
Mammalian (CLASP1/2) | COS-1, PTK1 | Transient | Akhmanova et al. 2001; Wittmann and Waterman-Storer 2005 |
| AtCLASP | Tobacco BY2 cells transgenic plants | Stable | Ambrose et al. 2007; Kirik et al. 2007 (YFP) | |
| EB1 | Mammalian (EB1) | LLCPK | Stable | Piehl and Cassimeris 2003; Salaycik et al. 2005 |
| Mammalian (EB1) | Xenopus A6 cells | Transient | Mimori-Kiyosue et al. 2000b | |
| Mammalian (EB3) | Neurons (Purkinje cells, mouse hippocampal neurons, mouse dorsal root ganglia neurons) | Transient (approaches include adenovirus virus infection) | Stepanova et al. 2003; Purro et al. 2008; Nakata and Hirokawa 2003 (YFP) | |
| Mammalian (EB3) | CHO-K1 | Transient | Komarova et al. 2009 (mCherry, mVenus) | |
| Mammalian (EB3) | Platelets (murine megakaryocytes) | Retroviral vector | Patel-Hett et al. 2008 | |
| Mammalian (EB3) | Goldfish fin fibroblasts (CCL71) | Transient | Efimov et al. 2008 (mCherry and CFP) | |
| Drosophila (EB1) | S2 cells | Stable | Rogers et al. 2008 (mRFP) | |
| Ustilago maydis (Peb1) | U. maydis | Stable (homologous recombination) | Straube et al. 2003; Fink and Steinberg 2006 (both YFP, RFP) | |
| S. cerevisiae (BIM1) | S. cerevisiae | Cen plasmid | Schwartz et al. 1997; Tirnauer et al. 1999; Cuschieri et al. 2006 | |
| S. pombe (Mal3) | S. pombe | Stable | Browning et al. 2003 | |
| Arabidopsis (AtEB1a,b) | Arabidopsis tissue-culture cells, tobacco BY-2 cells | Stable, transient | Chan et al. 2003; Van Damme et al. 2004; Dhonukshe et al. 2006; Kirik et al. 2007 (all CFP) | |
| Dictyostelium (DdEB1) | Dictyostelium | Transient | Rehberg and Graf 2002 | |
| Giardia (GlEB1) | Giardia | Episomal | Dawson et al. 2007 | |
| Ensconsin (also called E-MAP- 115, MAP7) | Mammalian (Ensconsin) | African green monkey/TC-7, human/MCF-7 | Stable | Faire et al. 1999; Bulinski et al. 2001 |
| Human EMTB (Ensconsin MT-binding domain) | Goldfish fin fibroblasts | Transient | Efimov et al. 2008 | |
| KAR9 | S. cerevisiae (KAR9) | S. cerevisiae | Cen plasmid | Miller and Rose 1998; Lee et al. 2000; Matheos et al. 2004; Cuschieri et al. 2006 |
| Katanin/Spastin | Mammalian (katanin, spastin) | CV-1, COS-7, HeLa, TC7, NIH 3T3 | Transient | Hartman and Vale 1999 (GFP, YFP, CFP); Buster et al. 2002; Errico et al. 2002; Evans et al. 2005, 2006 (last four YFP) |
| Caenorhabditis elegans (Mei-1) | HeLa | Transient | Srayko et al. 2000 | |
| Arabidopsis (AtKSS) | Arabidopsis | ~ | Bouquin et al. 2003 | |
| Tetrahymena (Kat1) | Tetrahymena | Stable | Sharma et al. 2007 | |
| MAP2 | Mammalian (MAP2) | HeLa, COS-7, Neuro-2A, cerebellar granule neurons | Transient | Ludin et al. 1996; Ozer and Halpain 2000; Björkblom et al. 2005 |
| MAP4 | Mammalian (MAP4) | BHK, CHO, Ltk | Stable, transient | Olson et al. 1995; Chang et al. 2001; Kremer et al. 2005 |
| Mammalian (MAP4) | Fava bean epidermal cells, cowpea mesophyll protoplasts, tobacco BY-2 cells | ~ | Marc et al. 1998; Dhonukshe and Gadella 2003; Dhonukshe et al. 2006; Dixit and Cyr 2003, 2004 (last two DsRed) | |
| Xenopus (XMAP4/p220) | Xenopus A6 cells | Stable | Shiina and Tsukita 1999 | |
| MAP65 | Arabidopsis (MAP65-1,4,5) | Tobacco BY-2 cells | Stable | Van Damme et al. 2004; Gaillard et al. 2008 |
| NuMa | Mammalian (NuMa) | HeLa | Transient | Merdes et al. 2000; Kisurina-Evgenieva et al. 2004 |
| NuSAP | Mammalian (NuSAP) | COS-1, PtK2 | Transient | Raemaekers et al. 2003 (GFP, YFP, and CFP) |
| P150 | Mammalian (rat brain p150) | COS-7 | Transient | Vaughan et al. 2002 (GFP and RFP) |
| Plakins | Human (ACF7) | Mouse visceral endoderm cells | Transient | Kodama et al. 2003 |
| Drosophila (short stop) | NIH-3T3, transgenic flies | Transient | Lee and Kolodziej 2002 | |
| Parkin co-regulated gene | Trypanosoma brucei (TbPACRG) | T. brucei | Stable | Dawe et al. 2005 |
| PRC1 | Mammalian (PRC1) | HeLa | Transient | Mollinari et al. 2002 |
| Stathmin | Xenopus stathmin | Xenopus A6 epithelial cells | Transient | Niethammer et al. 2004 (CFP and YFP) |
| Tea1 | S. pombe (Tea1) | S. pombe | Chromosomal integration | Behrens and Nurse 2002 (GFP, YFP); Feierbach et al. 2004 (YFP) |
| Tektin | Mouse (Tek2) | CHO | Stable | Durcan et al. 2008 |
| TOG/Dis1 | Xenopus (XMAP-215) | XL177 cells | Transient | Popov et al. 2001 |
| S. cerevisiae (Stu2) | S. cerevisiae | Stable | Kosco et al. 2001; Ma et al. 2007 (VFP) | |
| S. pombe (Dis1, Mtc1/Alp14) | S. pombe | Chromosomal integration | Nakaseko et al. 2001 (GFP and YFP) | |
| Dictyostelium (CP224) | Dictyostelium | Transient | Graf et al. 2000 | |
| tau | Bovine tau | B104, CHO, NIH-3T3, primary neurons, ES cells, transgenic mice | Transient, adenoviral, chromosomal integration | Illenberger et al. 1996; Ludin et al. 1996; Pratt et al. 2000 ; Lu and Kosik 2001; Stamer et al. 2002 (CFP). Many more recent include Jacobs et al. 2007; Glavaski-Joksimovic et al. 2009. |
| Bovine tau | Zebrafish | Embryo microinjection | Geldmacher-Voss et al. 2003 | |
| Mammalian | Xenopus embryo neonatal neurons | Embryo microinjection | Samsonov et al. 2004 | |
| Mammalian | Drosophila | Transgenic flies | Williams et al. 2000 | |
| Viral proteins | Herpes simplex VP-22 | COS-1 |
Transient | Martin et al. 2002 |
| Tobacco mosaic virus movement protein | COS-7 | Transient | Ferralli et al. 2006 | |
| The table is not exhaustive but is instead meant to provide information about the range of tools available. In many cases,
the paper cited is one of a series using the listed fusion or its variants. Inclusion is biased toward proteins that are known
to bind directly to MTs. For reasons of space limitation, motors and proteins with cellular localization limited to the centrosome
are excluded. (~) Indicates ambiguity in the primary source. |
||||
| (MBP) Microtubule-binding protein, (GFP) green fluorescent protein, (YFP) yellow fluorescent protein, (RFP) red fluorescent protein, (VFP) visual fluorescent protein. | ||||
