Recipe

Intracellular solution

0.4 mM GTP

4 mM Mg-ATP

2 mM MgCl2

140 mM potassium gluconate

10 mM TES

Adjust the osmolarity to 276 mOsm.

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  1. doi:10.1101/pdb.rec12388 Cold Spring Harb Protoc 2011: pdb.rec12388-
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