Polypeptone solution

1% polypeptone (pancreatic digest of casein)

0.2% NaCl (pH 7.2)

For 500 mL, solubilize 5 g of polypeptone and 1 g of NaCl in H₂O. Adjust the pH to 7.2 with 1 m NaOH, and bring to a total volume of 500 mL with H₂O. Sterilize by autoclaving and store at 4°C.

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