Formaldehyde (2% in PBS)
MATERIALS
Reagents
HCl (1 N)
Paraformaldehyde, electron microscopy (EM)-grade (Prill form, Electron Microscopy Sciences)
Equipment
Bottle, Pyrex, with cap
Fume hood
Hot plate
Stir bar, magnetic
METHOD
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1. Add the appropriate amount of EM-grade paraformaldehyde to PBS (e.g., 1 g of paraformaldehyde per 50 mL of PBS) in a Pyrex bottle with a stir bar.
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2. Heat the mixture on a hot plate in a hood with stirring at 60°C to dissolve the aldehyde.
Keep the bottle cap loosened so that pressure does not build up.
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3. Allow the solution to cool to 20°C, and adjust the pH to 7.4 with 1 N HCl.
- © 2011 Cold Spring Harbor Laboratory Press
