Recipe

Splicing buffer +ATP (5×)

175 mM KCl

20 mM MgCl2

10 mM ATP

100 mM creatine phosphate (disodium salt; Roche 10-621-714-001)

7.5 mM dithiothreitol (DTT)

250 μg/mL creatine kinase (Roche 10-127-566-001)

15% PEG 8000

Store at -70ºC to -80ºC.

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  1. doi:10.1101/pdb.rec12402 Cold Spring Harb Protoc 2011: pdb.rec12402-
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