Recipe

Splicing buffer +ATP (5×)

175 mM KCl

20 mM MgCl2

10 mM ATP

100 mM creatine phosphate (disodium salt; Roche 10-621-714-001)

7.5 mM dithiothreitol (DTT)

250 μg/mL creatine kinase (Roche 10-127-566-001)

15% PEG 8000

Store at -70ºC to -80ºC.

No Related Web Pages
| Table of Contents

This Article

  1. doi:10.1101/pdb.rec12402 Cold Spring Harb Protoc 2011: pdb.rec12402-
  1. » Full Text

Article Category

  1. Recipe

Personal Folder

  1. Save to Personal Folders

Updates/Comments

  1. Submit Updates/Comments
  2. No Updates/Comments published
  3. Alert me when Updates/Comments are published

Related Content

  1. Related Web Pages

Share

Current Issue

  1. April 2026, 2026 (4)
  1. Current Issue