Grape juice agar
MATERIALS
Reagents
24.0 g Bacto Agar
26.4 g sucrose
200 mL grape juice (100%)
g methylparaben
20 mL ethanol (95%)
Equipment
Beaker (large)
Microwave oven
Vials or plates (plastic)
METHOD
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1. Mix Bacto Agar and 800 mL water. Microwave until the agar dissolves completely (take care to prevent overflowing). Remove the solution from the microwave.
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2. Stir in sucrose and grape juice, and allow it to cool to 50°C.
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3. Dissolve methylparaben in 95% ethanol, and add to the agar solution. Stir well.
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4. Pour into plastic vials or plates (we typically use lids of Petri dishes), as desired. Let the agar cool and solidify before storing or using.
- © 2011 Cold Spring Harbor Laboratory Press
