Recipe

Normal Rat Ringer’s Solution

Reagent Concentration (mm) Amount for 1 L (g) Amount for 2 L (g)
NaCl 135 7.88 15.76
KCl 5.4 0.40 0.80
MgCl2·6H2O 1 0.204 0.408
CaCl2·2H2O 1.8 0.265 0.530
HEPES 5 1.19 2.38

  • Adjust pH to 7.2 with NaOH. Final osmolarity is ∼290 mOsm. This solution can be stored at 4°C for 1 mo.

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    1. doi:10.1101/pdb.rec068312 Cold Spring Harb Protoc 2012: pdb.rec068312- © 2012 Cold Spring Harbor Laboratory Press
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