Recipe

Voltage-Clamp Saline

150 mm NaCl

5 mm KCl

10 mm MgCl2

4 mm NaHCO3

0.5 mm EGTA

5 mm TES (2-[(2-hydroxy-1,1-bis(hydroxymethyl)ethyl)amino] ethanesulfonic acid)

5 mm trehalose

50 mm sucrose

Adjust to pH 7.2 with 1 n NaOH solution.

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  1. doi:10.1101/pdb.rec068320 Cold Spring Harb Protoc 2012: pdb.rec068320- © 2012 Cold Spring Harbor Laboratory Press

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