Voltage-Clamp Saline

150 mm NaCl

5 mm KCl

10 mm MgCl₂

4 mm NaHCO₃

0.5 mm EGTA

5 mm TES (2-[(2-hydroxy-1,1-bis(hydroxymethyl)ethyl)amino] ethanesulfonic acid)

5 mm trehalose

50 mm sucrose

Adjust to pH 7.2 with 1 n NaOH solution.

• © 2012 Cold Spring Harbor Laboratory Press