Recipe

Development Buffer for TIRFM

5 mm Na2HPO4

5 mm NaH2PO4

2 mm MgSO4

0.2 mm CaCl2

Dissolve the components in H2O and adjust the pH to 6.2. Sterilize by passing the solution through a filter (pore size, 0.23 µm).

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  1. doi:10.1101/pdb.rec068478 Cold Spring Harb Protoc 2012: pdb.rec068478- © 2012 Cold Spring Harbor Laboratory Press
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