CTAB (2%) Extraction Buffer
| Reagent | Quantity (for 30 mL) | Final concentration |
|---|---|---|
| Tris–HCl (1 m, pH 6) | 3 mL | 100 mm |
| NaCl (5 m) | 9 mL | 1.5 m |
| Hexadecyltrimethylammonium bromide (CTAB) (16%, w/v) | 3.75 mL | 2% (w/v) |
| EDTA (250 mm, pH 7) | 6 mL | 50 mm |
| DTT | 0.231 g | 50 mm |
- © 2012 Cold Spring Harbor Laboratory Press
