mPBS
| Reagent | Quantity (for 3.2 L) | Final concentration |
|---|---|---|
| NaCl (MW = 58.44) | 25.6 g | 136 mm |
| KCl (MW = 74.55) | 0.64 g (or 8.64 mL of 1 m stock) | 2.7 mm |
| KH2PO4 (MW = 136.09) | 0.7408 g | 1.7 mm |
| Na2HPO4 (MW = 141.96) | 3.648 g | 8.03 mm |
| Glycerol (MW = 92.10) (density = 1.261 g/cm3) | 160 mL | 685 mm |
| Sodium azide (MW = 65.02) | 3.2 g | 15.4 mm |
| BSA (MW = 67.000) | 3.2 g | 0.015 mm |
The pH should be 7 or slightly above. Adjust the volume to 3.2 L with distilled water. This solution is stable and can be used for several weeks.
- © 2012 Cold Spring Harbor Laboratory Press
