PHEM
| Reagent | Quantity (for 200 mL) | Final concentration |
|---|---|---|
| PIPES | 3.68 g | 60 mm |
| HEPES | 1.19 g | 21 mm |
| EGTA | 0.76 g | 10 mm |
| MgCl2 (1 m) | 400 µL | 2 mm |
| NaCl | 8 g | 685 mm |
Dissolve the reagents in distilled water and adjust the pH to 7.5 with KOH. Adjust the volume to 179 mL with distilled water. Add 1 mL of 25% glutaraldehyde and 20 mL of 32% paraformaldehyde. This solution works best if it is made fresh just before use.
- © 2012 Cold Spring Harbor Laboratory Press
