Recipe

Solution C

Reagent Quantity (for 1 L) Final concentration
NaCl (MW = 58.44) 5.8 g 100 mm
MgCl2 4.1 g 20 mm
KCl (MW = 74.55) 0.15 g 2 mm
BSA (MW = 67,000) 2 g 29.8 µm
2-(N-morpholino) ethanesulfonic acid (MES) (MW = 195.2) 2.13 g 0.01 mm
Sorbitol (MW = 182.17) 154.85 g 850 mm
EGTA (MW = 292.24) 0.38 g 1 mm

  • Adjust the pH to 5.8 with Tris base. Adjust the volume to 1 L with distilled water. Store in 50-mL aliquots at −20°C.

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    1. doi:10.1101/pdb.rec068841 Cold Spring Harb Protoc 2012: pdb.rec068841- © 2012 Cold Spring Harbor Laboratory Press
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