Embryo Embedding Agar
| Reagent | Amount to add |
|---|---|
| Low-melting-point agarose (Invitrogen) | 1 g |
| Sterile H2O | 100 mL |
| 100 mM HEPES (pH 7.3) | 50 µL |
| 8 mg/mL ethyl 3-aminobenzoate methanesulfonate (MS-222) (Sigma- Aldrich A5040) | 25 µL |
Melt at 50°C.
- © 2012 Cold Spring Harbor Laboratory Press
