Recipe

ACSF for Calcium Imaging

125 mm NaCl

26 mm NaHCO3

1.25 mm NaH2PO4

2.5 mm KCl

1.0 mm MgCl2

2.0 mm CaCl2

25 mm glucose

Bubble 95% O2 and 5% CO2 through the solution.

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  1. doi:10.1101/pdb.rec072959 Cold Spring Harb Protoc 2013: pdb.rec072959- © 2013 Cold Spring Harbor Laboratory Press
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