Recipe

Annealing Buffer (10×)

Reagent Quantity (for 1 mL) Final concentration (10×)
KCl (2 m) 375 µL 750 mm
Tris–HCl (1 m, pH 7.5) 200 µL 200 mm
H2O 425 µL –
Store indefinitely at –20°C.
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  1. doi:10.1101/pdb.rec073312 Cold Spring Harb Protoc 2013: pdb.rec073312- © 2013 Cold Spring Harbor Laboratory Press
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