Ca2+-Free Cytosol-Like Medium
| Reagent | Final concentration |
|---|---|
| KCl | 140 mm |
| NaCl | 20 mm |
| PIPES | 20 mm |
| EGTA | 1 mm* |
| MgCl2•6H2O | 2 mm |
| Adjust the pH to 7.0 with 5 m KOH. Take care when correcting the pH because the affinity of EGTA for Ca2+ is pH-sensitive. This solution is stable for months when stored at 4°C. | |
| *For convenience, use a 100 mm stock solution of EGTA. | |
- © 2013 Cold Spring Harbor Laboratory Press
