Buffer A for Yeast Extracts
| Reagent | Quantity (for 100 mL) | Final concentration |
|---|---|---|
| KCl (3 m) | 0.33 mL | 10 mm |
| HEPES (1 m, pH 7.9 at 25°C) | 1 mL | 10 mm (pH 7.0 at 4°C) |
| MgCl2 (1 m) | 0.15 mL | 1.5 mm |
| DTT (1 m) | 0.05 mL | 0.5 mm |
| PMSF (50 mm) | 1 mL | 0.5 mm |
| Leupeptin (1 mg/mL) | 0.1 mL | 1 µg/mL |
| Pepstatin (1 mg/mL) | 0.1 mL | 1 µg/mL |
| H2O | to 100 mL | |
| Add protease inhibitors just before use. Store at 4°C. Do not keep buffer to which protease inhibitors have been added for >1 wk. | ||
- © 2013 Cold Spring Harbor Laboratory Press
