Recipe

Denaturing Stop Buffer

Reagent Quantity (for 10 mL) Final concentration
NaOAc (3 m)   1 mL   0.3 m
SDS (10%)  0.2 mL   0.2%
EDTA (0.5 mm)   20 µL   1 mm
Proteinase K (10 mg/mL)   10 µL 10 µg/mL
H2O to 10 mL
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  1. doi:10.1101/pdb.rec078725 Cold Spring Harb Protoc 2013: pdb.rec078725- © 2013 Cold Spring Harbor Laboratory Press
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