Recipe

Native Gel-Loading Buffer

Reagent Quantity (for 10 mL) Final concentration
TBE (10×) 2.5 mL 2.5×
Glycerol (100%)  5 mL  50%
Bromophenol blue (1%)  1 mL  0.1%
H2O 1.5 mL
Take care to be accurate when measuring viscous solutions such as glycerol. Store at room temperature.
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  1. doi:10.1101/pdb.rec078733 Cold Spring Harb Protoc 2013: pdb.rec078733- © 2013 Cold Spring Harbor Laboratory Press

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