Splicing Buffer for Yeast Extracts (5×)
| Reagent | Quantity (for 100 µL) | Final concentration |
|---|---|---|
| Polyethylene glycol (PEG) 8000 (50%, w/v) (use highest quality) | 30 µL | 15% |
| KPO4 (0.6 m, pH 7.0) | 50 µL | 36 mm |
| Spermidine (100 mm) | 10 µL | 10 mm |
| MgCl2 (125 mm) | 10 µL | 12.5 mm |
| Take care to be accurate when measuring viscous solutions such as PEG. Make splicing buffer fresh to avoid precipitation of KPO4 on storage. | ||
- © 2013 Cold Spring Harbor Laboratory Press
