| Dulbecco’s minimal essential medium (DMEM) with high glucose and without l-glutamine, sodium pyruvate, or phenol red (Life Technologies 31053028)
|
48 mL |
|
| Fetal veal serum (FVS) |
5 mL |
10% (v/v) |
| HEPES (1 m)
|
1 mL |
20 mm |
| l-Glutamine (200 mm) (Life Technologies)
|
500 µL |
2 mm |
| Sodium pyruvate (100 mm) (Life Technologies)
|
500 µL |
1 mm |
|
|
|
| In cases of long observations, the medium can be supplemented with 1% (v/v) penicillin–streptomycin. Use a stock solution
containing 10,000 units of penicillin (base) and 10,000 µg of streptomycin (base)/mL utilizing penicillin G (sodium salt)
and streptomycin sulfate in 0.85% saline (Life Technologies). Imaging medium should be fresh or kept for no more than 2 d.
|
