Ni-NTA Lysis Buffer (5×)
| Reagent | Quantity (for 200 mL) |
Final concentration (5×) |
|---|---|---|
| Tris-HCl (1 m, pH 8.0) | 50 mL | 250 mm |
| NaCl (5 m) | 20 mL | 500 mm |
| Glycerol (100%) | 50 mL | 25% (v/v) |
| Autoclave. Store for up to 6 mo at 4°C. | ||
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