Recipe

Ni-NTA Lysis Buffer

Reagent Quantity (for 200 mL) Final concentration
β-Mercaptoethanol (14.1 m) 71 µL, add fresh      5 mm
Imidazole (1 m, pH 8.0)      200 µL 1 mm (pH 8.0)
ddH2O    to 200 mL
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  1. doi:10.1101/pdb.rec078923 Cold Spring Harb Protoc 2013: pdb.rec078923- © 2013 Cold Spring Harbor Laboratory Press
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