Inorganic Pyrophosphatase in Storage Buffer
| Reagent | Quantity (for 10 mL) | Final concentration |
|---|---|---|
| HEPES–KOH (1 m, pH 7.5) | 500 µL | 50 mm |
| DTT (2 m) | 5 µL | 1 mm |
| Glycerol, autoclaved | 5 mL | 50% |
| H2O | 4.45 mL | |
| Resuspend the IPP (Sigma I1891; 1000 units/mg, 100 µg/vial) in the solution above at a concentration of 0.5 unit/μL. Store it for up to 1 yr at −20°C. | ||
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