Modification Reaction Buffer A (Native)
| Reagent | Quantity (for 100 mL) (mL) |
Final concentration (mm) |
|---|---|---|
| HEPES/KOH (1 m, pH 7.5) | 1 | 10 |
| KCl (2 m) | 5 | 100 |
| MgCl2 (1 m) | 0.5 | 5 |
| H2O | 93.5 | |
| Adjust the MgCl2 concentration between 5 and 25 mm for individual substrates. Store indefinitely at 4°C. | ||
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